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KMID : 0377619760300060725
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Korean Jungang Medical Journal
1976 Volume.30 No. 6 p.725 ~ p.730
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Urinary. Amino Acid Pattern in Various Diseases of Children
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Abstract
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In order to observe the changes of urinary amino acid excretions under the conditions of several diseases, such as diphtheria, poliomyelitis, measles, nephritis pneumonia and purpura, commonly occurred in children, the author designed, to perform the present investigation.
The urine samples used in he experiment were those of 100ml aliquots, of the total volume of 24-hour urine collected from 19 individual patients.
The urinary, amino acid assays, using with, twice dilutions of the samples, were carried out by detecting the , colored, ninhydrine , spots of`amino acids isolated on the papers after 10 hour development by chromatography; with both one dimensional and two-dimensional ascending methods using butanol-acetic acid-water solvent system. Widths and color densities of the spots obtained from urine of the patients could be evaluated by comparing with those of control normal subjects.
The results obtained in the investigation were as follows.￢
1). Small amount of free amino acids were excreted in the urine of diphtheria patients, but it was shown characteristics by excreting leucine in larger amounts compared, with those of control normal subjects.
2). There were no differences between the urine of poliomyelitis and the controls.
3). In measles, more amino acids were excreted than those of the controls, especially leucine, isoleucine, phenylalanine, valine, tryptophane and tyrosine.
4). Tryptophane was characteristically occurred in case of nephritis, while
others were, shown scarcely.
5). More amounts of glutamic acid, threonine and argnine were excreted in the urine of purpura patients.
6). In pneumonia, the urine contained more quantities of lysine anti & histidine, while others similarly to those of the controls.
Considering the results of above observations, the author came to the conclusion , that the urinary free amino acid pattern were different from one another in accordance with the diseases.
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